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Made in U.S.A
Models:
334
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Product Introduction:
This hands-on VNTR Human DNA Typing kit teaches DNA fingerprinting at the D1S80 locus on chromosome 1, which contains 14–40 tandem repeats of a 16 bp sequence. Students will extract their own DNA (or hair-derived DNA per addendum), set up PCR with EdvoBeads™, amplify the VNTR region, and visualize allele sizes via agarose gel electrophoresis.
Group Size:
For 25 students
Time Required:
• DNA Extraction: 30 minutes
• PCR Setup: 10 minutes
• PCR Run: 90 minutes
• Electrophoresis: 30 minutes
• Gel Staining: 5 minutes
Kit Includes:
• Instructions (with hair-DNA isolation addendum)
• PCR EdvoBeads™
• LyphoPrimer™ mix for D1S80 locus
• EdvoQuick™ DNA Ladder
• LyphoControl™ DNA sample
• Universal DNA Buffer
• TE Buffer
• Proteinase K
• UltraSpec-Agarose™
• TBE Electrophoresis Buffer powder
• SYBR® Safe Stain
• Microcentrifuge tubes
• PCR tubes
• Disposable plastic cups
• Salt packets
• 15 mL conical tube
All You Need:
• Thermal cycler or water baths
• DNA electrophoresis apparatus
• Micropipettes (5–50 µL) and tips
• UV or blue-light transilluminator
• Microcentrifuge
• Microwave or hot plate
• Distilled water
Storage:
Some components require freezer storage upon receipt.