MAST BACTERURITEST STRIPS, 5x 200 Strips

MAST® BACTERURITEST STRIPS — BTR1

Intended use
A strip test for the detection of bacteriuria.

FOR IN VITRO DIAGNOSTIC USE ONLY

Material
MAST® BACTERURITEST Strips are 75 mm long by 6 mm wide, marked and prefolded 12 mm from one end and printed “BACTERURITEST: DIP TO LINE”. They are packed with the prefolded end down to simplify handling.

Storage and shelf life
All containers should be kept tightly closed and stored in a dry place at 25°C or below until the expiry date shown on the pack label.

Precautions

• For in vitro diagnostic use only.
• Observe approved hazard precautions and aseptic techniques.
• To be used only by adequately trained and qualified laboratory personnel.
• Sterilise all biohazard waste before disposal.
• Refer to Product Safety Data sheet (available on request or via MAST® website).

Materials required but not provided
Standard microbiological supplies and equipment such as loops, MAST® dehydrated culture media, swabs, applicator sticks, incinerators and incubators, etc.

Procedure

1. Urines should be sampled with a minimum of delay after collection unless a reliable method of preservation, such as refrigeration, is employed.
2. Dip a MAST® BACTERURITEST Strip to the mark in the thoroughly mixed sample of urine, remove, and allow any excess urine to be absorbed.
3. Apply the area of the strip below the mark to the surface of a well-dried, solid culture medium plate, such as MAST® C.L.E.D. Medium (DM110D) or MAST® C.L.E.D. with Andrade’s Indicator (DM111D).
4. Ensure the whole of the inoculum area is in contact with the surface of the medium and leave in contact for 2 to 3 seconds before removing. Up to 14 urines may be conveniently inoculated onto a 90 mm petri dish.
5. Incubate aerobically at 35–37°C for 18–24 hours.

Interpretation of results

• After incubation count and record the number of colonies growing in the inoculated area.
• Two colony count levels represent 100,000 or more organisms per mL.
• For bacilli: a colony count of 25 or more indicates significant bacteriuria.
• For cocci: a colony count of 30 or more indicates significant bacteriuria.
• It is suggested that a colony count of 20 or more, regardless of type, should be considered significant.
• Bacterial populations of fewer than 10,000 organisms per mL give few colonies and frequently give no growth.

Quality control

• Check for signs of deterioration.
• Quality control must be performed with at least one organism to demonstrate expected performance.
• Do not use the product if the result with the control organism is incorrect.
• Suggested performance control strains: